Informace o publikaci

Identification of Nucleolus-Associated Chromatin Domains Reveals a Role for the Nucleolus in 3D Organization of the A. thaliana Genome

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PONTVIANNE Frédéric N. CARPENTIER Marie-Christine DURUT Nathalie PAVLIŠTOVÁ Veronika JAŠKE Karin SCHOŘOVÁ Šárka PARRINELLO Hugues ROHMER Marine PIKAARD Craig S. FOJTOVÁ Miloslava FAJKUS Jiří SAEZ-VASQUEZ Julio Herman

Rok publikování 2016
Druh Článek v odborném periodiku
Časopis / Zdroj Cell Reports
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://www.sciencedirect.com/science/article/pii/S2211124716309020
Doi http://dx.doi.org/10.1016/j.celrep.2016.07.016
Obor Genetika a molekulární biologie
Klíčová slova NUCLEAR LAMINA INTERACTIONS; TRANSFER-RNA GENES; ARABIDOPSIS INTERPHASE NUCLEI; DOSAGE CONTROL; 45S RDNA; CHROMOSOMES; TELOMERES; TRANSCRIPTION; DYNAMICS; STATES
Popis The nucleolus is the site of rRNA gene transcription, rRNA processing, and ribosome biogenesis. However, the nucleolus also plays additional roles in the cell. We isolated nucleoli using fluorescence-activated cell sorting (FACS) and identified nucleolus-associated chromatin domains (NADs) by deep sequencing, comparing wild-type plants and null mutants for the nucleolar protein NUCLEOLIN 1 (NUC1). NADs are primarily genomic regions with heterochromatic signatures and include transposable elements (TEs), sub-telomeric regions, and mostly inactive protein-coding genes. However, NADs also include active rRNA genes and the entire short arm of chromosome 4 adjacent to them. In nuc1 null mutants, which alter rRNA gene expression and overall nucleolar structure, NADs are altered, telomere association with the nucleolus is decreased, and telomeres become shorter. Collectively, our studies reveal roles for NUC1 and the nucleolus in the spatial organization of chromosomes as well as telomere maintenance.
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