Informace o publikaci

Polymer Pencil Graphite Electrode in the Study of Electrochemical Processes of Guanine Analogues



Rok publikování 2017
Druh Článek ve sborníku
Konference 68th Annual Meeting of the International Society of Electrochemistry
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Obor Fyzikální chemie a teoretická chemie
Klíčová slova guanine; polymer pencil graphite electrode; cyclic volatmmetry; electron microscopy; Raman spectroscopy
Popis Understanding the oxidation processes of purines and their derivatives is primary for the improvement of electrochemical methods of genetic and food materials analysis. However, it is generally known that guanine is one of the four main nucleobases found in the nucleic acids DNA and RNA, but it is less known that G, G-based purines, including the nucleoside and the nucleotide monophosphate play an important role in diet-related diuseases, helath of cardiovascular nad digestive systems and in neurodegenerative diseases. Electrochemical techniques in connection with carbon electrodes are powerful methods for analysis of G and its derivatives. Our previous research revelaed that a polymer pencil graphite electrode can serve as a sensitive and disposable sensor for purines, including G and its derivatives. In this work, the electrochemical oxidation processes of G, guanosine and guanosine monophosphate and their deoxy-derivatives were investigated. The dependence of oxidation signals on experimental conditions such as scan rate, electrode surface pretreatment, addition of Cu(II) ions, adsorption, pH and the presence of structural increments (ribofuranosyl-, deoxyribofuranosyl-, and phosphate group) alolowed us to evaluate:(i) kinetics and mechanism of G and GMP oxidation (electron and proton numbers, coefficients of electro-transfer, the role of electrode surface), (ii) the effect of adsorption and (iii) the effect of oxo, deoxy, and phosphate groups on oxidation responses. We can conclude that polymer pencil lead is a prospective electrode material with good stability and reproducibility not only for analysis of G and its analogues but also for whole purine group.
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