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Carboxyl-rich plasma polymer surfaces in surface plasmon resonance immunosensing

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MAKHNEVA Ekaterina OBRUSNÍK Adam FARKA Zdeněk SKLÁDAL Petr VANDENBOSSCHE Marianne HEGEMANN Dirk ZAJÍČKOVÁ Lenka

Druh Článek v odborném periodiku
Časopis / Zdroj Japanese Journal of Applied Physics
Fakulta / Pracoviště MU

Přírodovědecká fakulta Středoevropský technologický institut

Citace
Doi http://dx.doi.org/10.7567/JJAP.57.01AG06
Obor Fyzika plazmatu a výboje v plynech
Klíčová slova carboxyl-rich plasma polymer; immobilization; surface plasmon resonance; immunosensor
Popis Stable carboxyl-rich plasma polymers (PPs) were deposited onto the gold surface of surface plasmon resonance (SPR) chips under conditions that were chosen based on lumped kinetic model results. Carboxyl-rich films are of high interest for bio-applications thanks to their high reactivity, allowing the formation of covalent linkages between biomolecules and a surface. Accordingly, the monoclonal antibody, specific to human serum albumin (HSA), was immobilized and the performance of SPR immunosensors was evaluated by the immunoassay flow test. The developed sensors performed high level of stability and provided selective and high response to the HSA antigen solutions. The achieved results confirmed that the presented methodologies for the grafting of biomolecules on the gold surfaces have great potential for biosensing applications.
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