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An improved design to capture magnetic microparticles for capillary electrophoresis based immobilized microenzyme reactors

Název česky Vylepšený design pro spojeni CE IMER
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RAMANA Pranov SCHEJBAL Jan HOUTHOOFD Kristof MARTENS Johan ERWIN Adams AUGUSTIJNS Patrick GLATZ Zdeněk VAN SCHEPDAEL Ann

Rok publikování 2018
Druh Článek v odborném periodiku
Časopis / Zdroj Electrophoresis
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Doi http://dx.doi.org/10.1002/elps.201700434
Obor Biochemie
Klíčová slova IMER; Magnetic microparticles; Immobilization; In-line capillary electrophoresis
Popis In this paper, we demonstrate the effectiveness of a new 3D printed magnet holder that enables capture of magnetic microparticles in commercially available capillary electrophoresis equipment with a liquid or air based coolant system. The design as well as the method to capture magnetic microparticles inside the capillary are discussed. This setup was tested at temperature and pH values suitable for performing enzymatic reactions. To demonstrate its applicability in CE- immobilized microenzyme reactors (IMER) development, human flavin-containing monooxygenase 3 and bovine serum albumin were immobilized on amino functionalized magnetic microparticles using glutaraldehyde. These microparticles were subsequently used to perform in-line capillary electrophoresis with clozapine as a model substrate.
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