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The inositol phosphatase SHIP2 enables sustained ERK activation downstream of FGF receptors by recruiting Src kinases

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FAFÍLEK Bohumil BÁLEK Lukáš BOSÁKOVÁ Michaela VAŘECHA Miroslav NITĂ Alexandru GREGOR Tomáš GUDERNOVÁ Iva KŘENOVÁ Jitka GHOSH S. PISKÁČEK Martin JONATOVA Lucie ČERNOHORSKÁ Nicole ZIEBA J.T. KOSTAS M. HAUGSTEN E.M. WESCHE J. ERNEUX C. TRANTÍREK Lukáš KRAKOW D. KREJČÍ Pavel

Rok publikování 2018
Druh Článek v odborném periodiku
Časopis / Zdroj SCIENCE SIGNALING
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1126/scisignal.aap8608
Klíčová slova The inositol phosphatase SHIP2
Popis Sustained activation of extracellular signal-regulated kinase (ERK) drives pathologies caused by mutations in fibroblast growth factor receptors (FGFRs). We previously identified the inositol phosphatase SHIP2 (also known as INPPL1) as an FGFR-interacting protein and a target of the tyrosine kinase activities of FGFR1, FGFR3, and FGFR4. We report that loss of SHIP2 converted FGF-mediated sustained ERK activation into a transient signal and rescued cell phenotypes triggered by pathologic FGFR-ERK signaling. Mutant forms of SHIP2 lacking phosphoinositide phosphatase activity still associated with FGFRs and did not prevent FGF-induced sustained ERK activation, demonstrating that the adaptor rather than the catalytic activity of SHIP2 was required. SHIP2 recruited Src family kinases to the FGFRs, which promoted FGFR-mediated phosphorylation and assembly of protein complexes that relayed signaling to ERK. SHIP2 interacted with FGFRs, was phosphorylated by active FGFRs, and promoted FGFR-ERK signaling at the level of phosphorylation of the adaptor FRS2 and recruitment of the tyrosine phosphatase PTPN11. Thus, SHIP2 is an essential component of canonical FGF-FGFR signal transduction and a potential therapeutic target in FGFR-related disorders.
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