Informace o publikaci

Lectin PLL3, a Novel Monomeric Member of the Seven-Bladed beta-Propeller Lectin Family

Název česky Lektin PLL3, nový monomerní člen lektinové rodiny sedmi-lopatkových beta-vrtulí
Autoři

FALTINEK Lukáš FUJDIAROVÁ Eva MELICHER Filip HOUSER Josef KAŠÁKOVÁ Martina KONDAKOV Nikolay KONONOV Leonid PARKAN Kamil VIDAL Sébastien WIMMEROVÁ Michaela

Rok publikování 2019
Druh Článek v odborném periodiku
Časopis / Zdroj Molecules
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www Full Text
Doi http://dx.doi.org/10.3390/molecules24244540
Klíčová slova Photorhabdus; lectin; O-methylated saccharides; L-fucose
Popis The Photorhabdus species is a Gram-negative bacteria of the family Morganellaceae that is known for its mutualistic relationship with Heterorhabditis nematodes and pathogenicity toward insects. This study is focused on the characterization of the recombinant lectin PLL3 with an origin in P. laumondii subsp. laumondii. PLL3 belongs to the PLL family of lectins with a seven-bladed beta-propeller fold. The binding properties of PLL3 were tested by hemagglutination assay, glycan array, isothermal titration calorimetry, and surface plasmon resonance, and its structure was determined by X-ray crystallography. Obtained data revealed that PLL3 binds similar carbohydrates to those that the other PLL family members bind, with some differences in the binding properties. PLL3 exhibited the highest affinity toward L-fucose and its derivatives but was also able to interact with O-methylated glycans and other ligands.
Související projekty: