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KIF14 controls ciliogenesis via regulation of Aurora A and is important for Hedgehog signaling

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PEJŠKOVÁ Petra REILLY Madeline Louise BINÓ Lucia BERNATÍK Ondřej DOLANSKÁ Linda GANJI Sri Ranjani ZDRÁHAL Zbyněk BENMERAH Alexandre ČAJÁNEK Lukáš

Rok publikování 2020
Druh Článek v odborném periodiku
Časopis / Zdroj JOURNAL OF CELL BIOLOGY
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://rupress.org/jcb/article/219/6/e201904107/151721/KIF14-controls-ciliogenesis-via-regulation-of?searchresult=1
Doi http://dx.doi.org/10.1083/jcb.201904107
Klíčová slova PRIMARY CILIUM; MOTHER CENTRIOLE; MOTOR PROTEINS; SONIC HEDGEHOG; EARLY STEPS; KINESIN-II; CELL-CYCLE; A KINASE; ACTIVATION; CEP164
Popis Primary cilia play critical roles in development and disease. Their assembly and disassembly are tightly coupled to cell cycle progression. Here, we present data identifying KIF14 as a regulator of cilia formation and Hedgehog (HH) signaling. We show that RNAi depletion of KIF14 specifically leads to defects in ciliogenesis and basal body (BB) biogenesis, as its absence hampers the efficiency of primary cilium formation and the dynamics of primary cilium elongation, and disrupts the localization of the distal appendage proteins SCLT1 and FBF1 and components of the IFT-B complex. We identify deregulated Aurora A activity as a mechanism contributing to the primary cilium and BB formation defects seen after KIF14 depletion. In addition, we show that primary cilia in KIF14-depleted cells are defective in response to HH pathway activation, independently of the effects of Aurora A. In sum, our data point to KIF14 as a critical node connecting cell cycle machinery, effective ciliogenesis, and HH signaling.
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