Informace o publikaci

Cell Proliferation of HaCaT Keratinocytes on Collagen Films Modified by Argon Plasma Treatment

Autoři

GARCIA Jorge Lopez ASADINEZHAD Ahmad PACHERNÍK Jiří LEHOCKÝ Marián JUNKAR Ita HUMPOLÍČEK Petr SÁHA Petr VALASEK Pavel

Rok publikování 2010
Druh Článek v odborném periodiku
Časopis / Zdroj Blood Cells, Molecules and Diseases
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://www.mdpi.com/1420-3049/15/4/2845
Doi http://dx.doi.org/10.3390/molecules15042845
Klíčová slova plasma treatment; atelocollagen; surface modification; HaCaT; cell proliferation
Popis Argon plasma treatment was used to modify the surface of atelocollagen films using a plasmochemical reactor. To evaluate the effects of the treatment, the untreated and treated samples were characterized by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), Scanning Electron Microscopy (SEM) imaging, and X-ray Photoelectron Spectroscopy (XPS) techniques. Cell growth was carried out by culturing human immortalized keratinocyte (HaCaT) cells and proliferation was measured via MTT assay. It was observed that argon plasma treatment significantly enhanced the extent of cell proliferation, which was ascribed to the favourable role of plasma treatment in inducing surface oxygen-containing entities together with increasing surface roughness. This can be considered as a potentially promising approach for tissue regeneration purposes.