Informace o publikaci
D-Lactate Dehydrogenase (Cytochrome) from Saccharomyces cerevisiae Purification by Fast Protein Liquid Chromatography
| Autoři | |
|---|---|
| Rok publikování | 2009 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Polish journal of chemistry |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://www.ichf.edu.pl/pjch/pj-2009.htm |
| Klíčová slova | baker's yeast; mitochondria; d-lactic acid; isolation |
| Popis | D-Lactate dehydrogenase (cytochrome) was isolated from baker's yeasts (Saccharomyces cerevisiae) using fast protein liquid chromatography. Mitochondria from disrupted cells were separated and enzymes released by Triton X-100. Purification was performed by fast protein liquid chromatography on hydroxylapatite and in another separate experiment on AH-Sepharose with covalently bound 4-hydroxy-alpha cyanocinnamic acid (SHCA). Although purification on hydtoxylapatite provided approachable samples with activities for only D-lactic acid, chromatography on SHCA seemed to be better because a purified fraction of enzyme with the specific activity of 3.38 nkat/mg was obtained. Another problem considered was the usefulness of the combination of chromatography on hydroxylapatite and SHCA providing a fraction of D-lactic acid with specific activity of 5.71 nkat/mg and no cross reactivity for L-lactic acid. |