Informace o publikaci

Characterization and in vitro Analysis of Probiotic-Derived Peptides Against Multi Drug Resistance Bacterial Infections

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MAZUMDAR Aninda HADDAD Yazan SUR Vishma Pratap MILOSAVLJEVIC Vedran BHOWMICK Sukanya MICHÁLKOVÁ Hana GURÁŇ Roman VESELÝ Radek MOULICK Amitava

Rok publikování 2020
Druh Článek v odborném periodiku
Časopis / Zdroj Frontiers in Microbiology
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://www.frontiersin.org/articles/10.3389/fmicb.2020.01963/full
Doi http://dx.doi.org/10.3389/fmicb.2020.01963
Klíčová slova antibacterial peptides antibiotics multidrug resistance bacteria infections
Popis An inexorable switch from antibiotics has become a major desideratum to overcome antibiotic resistance. Bacteriocin fromLactobacillus casei, a cardinal probiotic was used to design novel antibacterial peptides named as Probiotic Bacteriocin Derived and Modified (PBDM) peptides (PBDM1: YKWFAHLIKGLC and PBDM2: YKWFRHLIKKLC). The loop-shaped 3D structure of peptides was characterizedin silicovia molecular dynamics simulation as well as biophysically via spectroscopic methods. Thereafter,in vitroresults against multidrug resistant bacterial strains and hospital samples demonstrated the strong antimicrobial activity of PBDM peptides. Further,in vivostudies with PBDM peptides showed downright recovery of balb/c mice from Vancomycin ResistantStaphylococcus aureus(VRSA) infection to its healthy condition. Thereafter,in vitrostudy with human epithelial cells showed no significant cytotoxic effects with high biocompatibility and good hemocompatibility. In conclusion, PBDM peptides displayed significant antibacterial activity against certain drug resistant bacteria which cause infections in human beings. Future analysis are required to unveil its mechanism of action in order to execute it as an alternative to antibiotics.
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