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Making good’s buffers good for freezing: The acidity changes and their elimination via mixing with sodium phosphate

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VESELÝ Lukáš SUSRISWETA Behera HEGER Dominik

Rok publikování 2021
Druh Článek v odborném periodiku
Časopis / Zdroj International Journal of Pharmaceutics
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://doi.org/10.1016/j.ijpharm.2020.120128
Doi http://dx.doi.org/10.1016/j.ijpharm.2020.120128
Klíčová slova pH shift; Cryopreservation; Stabilization; Freeze-concentrated solution; Sulfonephthalein indicators; Hammett acidity function; Cooling rate
Popis Solutions of three Good’s buffers (HEPES, MOPS, and MES), both pure and mixed with sodium phosphate buffers (Na-P), are investigated in terms of the freezing-induced acidity changes in their operational pH ranges. The Good’s buffers have the tendency to basify upon freezing and, more intensively, at lower pHs. The acidity varies most prominently in MES, where the change may reach the value of two. Importantly, the Good’s buffers are shown to mitigate the strong acidification in the Na-P buffer. Diverse concentrations of the Good’s buffers are added to cancel out the strong, freezing-induced acidity drop in 50 mM Na-P that markedly contributes to the solution’s acidity; the relevant values are 3 mM HEPES, 10 mM MOPS, and 80 mM MES. These buffer blends are therefore proposed to be applied in maintaining approximately the acidity of solutions even after the freezing process and, as such, should limit the stresses for frozen chemicals and biochemicals.
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