Informace o publikaci

Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy

Logo poskytovatele
Autoři

TURČANOVÁ Michaela HRTOŇ Martin DVOŘÁK Petr NOVÁK Kamil HERMANOVÁ Markéta BEDNAŘÍK Zdeněk POLZER Stanislav BURŠA Jiří

Rok publikování 2021
Druh Článek v odborném periodiku
Časopis / Zdroj BioMed Research International
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://www.hindawi.com/journals/bmri/2021/6879765/
Doi http://dx.doi.org/10.1155/2021/6879765
Klíčová slova Full-Range Optical Imaging; Planar Collagen Fiber; Polarized Light Microscopy
Popis A novel method for semiautomated assessment of directions of collagen fibers in soft tissues using histological image analysis is presented. It is based on multiple rotated images obtained via polarized light microscopy without any additional components, i.e., with just two polarizers being either perpendicular or nonperpendicular (rotated). This arrangement breaks the limitation of 90° periodicity of polarized light intensity and evaluates the in-plane fiber orientation over the whole 180° range accurately and quickly. After having verified the method, we used histological specimens of porcine Achilles tendon and aorta to validate the proposed algorithm and to lower the number of rotated images needed for evaluation. Our algorithm is capable to analyze 5·105 pixels in one micrograph in a few seconds and is thus a powerful and cheap tool promising a broad application in detection of collagen fiber distribution in soft tissues.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.

Další info