Informace o publikaci

Kinetics of platelet adhesion to a fibrinogen-coated surface in whole blood under flow conditions

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GABBASOV Z. A. AVTAEVA Y. N. MELNIKOV I. S. OKHOTA S. D. CAPRNDA M. MOZOS I. PROSECKÝ Robert RODRIGO L. KRUŽLIAK Peter ZOZULYA N. I.

Rok publikování 2021
Druh Článek v odborném periodiku
Časopis / Zdroj JOURNAL OF CLINICAL LABORATORY ANALYSIS
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
www https://onlinelibrary.wiley.com/doi/10.1002/jcla.23939
Doi http://dx.doi.org/10.1002/jcla.23939
Klíčová slova fibrinogen-coated surface; flow conditions; platelet adhesion; recording of a scattered laser light signal from a fibrinogen-covered surface; whole blood
Popis Aim To test a novel method of assessment of platelet adhesion to a fibrinogen-coated surface in whole blood under flow conditions. Methods We developed a fluidic device that mimics blood flow in vessels. The method of detection of platelet adhesion is based on recording of a scattered laser light signal from a fibrinogen-covered surface. Testing was performed in platelet-rich plasma (PRP) and whole blood of healthy volunteers. Control measurements were performed, followed by tests with inhibition of platelet GPIIa/IIIb and GPIb receptors. Then, the same testing sequence was performed in whole blood of persons with autoimmune thrombocytopenia and type 3 von Willebrand disease. Results The change in intensity of scattered light was 2.7 (2.4; 4.1) times higher in whole blood (0.2 +/- 0.08V, n = 7) than in PRP (0.05 +/- 0.02 V, n = 7), p < 0.01. The blocking of GP IIb/IIIa receptors decreased the intensity of scattered light to 8.5 (6.5;12)%; the blocking of GPIb receptors decreased it to 34 (23;58)%, p < 0.01. In the whole blood of a person with autoimmune thrombocytopenia, the inhibition of GPIb receptors decreased platelet adhesion, but no effect was observed in type 3 von Willebrand disease. Inhibition of platelet GPIIb/IIIa receptors alone or combined inhibition of GPIb and GPIIb/IIIa receptors resulted in almost total suppression of adhesion in both cases. Conclusion Our system effectively registers platelet adhesion to a fibrinogen-coated surface under controlled-flow conditions and may successfully be applied to the investigation of platelet adhesion kinetics.

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