Informace o publikaci

HILIC Separation Methods on Poly-Hydroxyl Stationary Phases for Determination of Common Saccharides with Evaporative Light-Scattering Detector and Rapid Determination of Isomaltulose in Protein-Rich Food Supplements

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CRHA Tomáš ODEDINA Grace F PAZOUREK Jiří

Rok publikování 2024
Druh Článek v odborném periodiku
Časopis / Zdroj Fluid - Particle Separations Journal
Fakulta / Pracoviště MU

Farmaceutická fakulta

Citace
www https://www.mdpi.com/2297-8739/11/2/45
Doi http://dx.doi.org/10.3390/separations11020045
Klíčová slova HILIC; ELSD; saccharides; sugar alcohols; isomaltulose (Palatinose); food supplements
Popis This article highlights the fundamental aspects of hydrophilic interaction liquid chromatography (HILIC) on poly-hydroxyl stationary phases to analyze non-derivatized mono- and disaccharides, including commonly consumed carbohydrates like glucose, fructose, sucrose, and lactose. The evaporative light-scattering detector (ELSD) is utilized as an alternative to an MS detector, and the separation system's selectivity allows the separation of anomers of monosaccharides. The study also includes a rapid method for determining isomaltulose (Palatinose), which was validated and applied to food supplement samples available in the Czech market, even those with high protein content. Additionally, isomaltulose was separated from sucrose in just 13 min.
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