Informace o publikaci
Revealing Hard Tissue Adaptation and Remodeling Dynamics with BEE-ST: Novel Spatiotemporal Imaging Approach
| Autoři | |
|---|---|
| Rok publikování | 2025 |
| Druh | Vyžádané přednášky |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Understanding biomineralisation—the process driving the formation of hard tissues such as bone and teeth—is key to revealing their unique mechanical properties, inspiring biomimetic materials for tissue repair, and uncovering how ageing and disease affect skeletal health. While techniques like micro-computed tomography (µCT) and standard histological staining provide insights into biomineralisation-associated processes, they typically capture only static endpoints, leaving the dynamic, time-dependent morphological changes largely unresolved. To overcome these limitations, we employed the BEE-ST (Bones and tEEth Spatio-Temporal growth monitoring) approach, developed in our laboratory, which combines state-of-the-art imaging with signal intensity quantification to enable precise, cross-species tracking of development, remodeling, regeneration, and healing across a wide range of calcified tissues. For the first time, this allowed a detailed spatiotemporal analysis of bone remodeling after physical exercise and adaptation to reduced mechanical load, revealing how these conditions affect bone morphology, structural integrity, and remodeling dynamics. To complement these findings, high-resolution µCT was used to quantify architectural changes and infer mechanical properties. This highly reproducible approach offers a novel way to explore the biomineralisation process in greater depth, providing valuable insights for clinical applications and advancing research in hard tissue biology. |