Informace o publikaci
D-AMINO ACIDS IN THE LIMELIGHT: ENANTIOSEPARATION OF AMINO ACIDS USING CHIRAL STATIONARY PHASE BY LC-MS
| Autoři | |
|---|---|
| Rok publikování | 2025 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | In the past, D-amino acids, enantiomeric counterparts of proteinogenic L-amino acids, were deemed as unnatural and even non-functional. However, studies in the last decades indicate their role as a novel class of signaling molecules in many organs. Free D-amino acids, namely D-Serine, D-Aspartate, and D-Cysteine play vital roles in brain physiology, therefore alterations in their levels appear to be linked to numerous neurological, neurodegenerative, and psychiatric disorders. Our project aims to develop robust LC-MS platform for the quantification of D- and L- amino acids in biological fluids, i.e., blood plasma. For the method development, we used CROWNPAC CR-I(-) column with a chiral crown ether-bonded stationary phase, and mobile phase with a high percentage of organic solvent. Precise and accurate analytical platform is crucial for a better understanding of the role of D-amino acids in the neurobiology of disorders such as schizophrenia, major depressive disorders, bipolar disorder, autism spectrum disorders or amyotrophic lateral sclerosis. In addition, it may offer the identification of novel biomarkers and/or monitoring of the progression of disease. |
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