Informace o publikaci
ZnO nanoparticle effects on Arabidopsis thaliana: Insights into miRNA and metabolite profiles
| Autoři | |
|---|---|
| Rok publikování | 2025 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | GENE REPORTS |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | https://linkinghub.elsevier.com/retrieve/pii/S2452014425002250 |
| Doi | https://doi.org/10.1016/j.genrep.2025.102352 |
| Klíčová slova | Arabidopsis thaliana; ZnO nanoparticles; Toxicity; miRNA; Secondary metabolites |
| Popis | The application of zinc oxide nanoparticles (ZnO NPs) in agriculture and plant research is a topic of significant debate. In this study, we explored the molecular responses of Arabidopsis thaliana (Columbia (Col-0) ecotype) to ZnO NP exposure, with a primary focus on miRNA expression and metabolite profiles, providing new insights into the mechanisms underlying plant responses. ZnO NPs were applied at three concentrations (8, 40, 80 mg center dot kg-1) in peat tablets (Jiffy (R)). Our results reveal dose-dependent negative effects on plant phenotypic traits, particularly reductions in growth and chlorophyll content. Notably, ZnO NPs caused significant disruptions in metal homeostasis, with elevated zinc accumulation and altered uptake of manganese, iron, and calcium. Gene expression analysis of antioxidant defense responses demonstrated concentration-specific regulation, indicating a shift towards more specialized antioxidants under ZnO NP stress. Specifically, the lower concentrations (8 and 40 mg center dot kg-1) led to the overexpression of genes related to H2O2 scavenging and glutathione synthesis, while the 80 mg center dot kg-1 concentration induced the upregulation of genes associated with superoxide scavenging, highlighting a dose-dependent shift in oxidative stress response mechanisms. These molecular changes were accompanied by significant alterations in miRNA expression and metabolite profiles, in a non-linear and dose-dependent manner. A significant role was observed for the miR156-SPL regulatory module at the 40 and 80 mg center dot kg-1 concentrations. Furthermore, proline (approx. 20 % increase, 8, 80 mg center dot kg-1), taurine (16 %, 80 mg center dot kg-1), fumaric acid (31 %, 40 mg center dot kg-1), and glutamic acid (50 %, 8 mg center dot kg-1) were implicated in the plant's adaptive response to ZnO NPs, contributing to antioxidant defense and metal-binding mechanisms. Together, these results highlight that ZnO NP exposure elicits complex and multi-layered adaptive mechanisms involving the interplay of elemental stress, antioxidant responses, miRNA regulation, and metabolic adjustments. |