Informace o publikaci
Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles
| Autoři | |
|---|---|
| Rok publikování | 2001 |
| Druh | Článek ve sborníku |
| Konference | The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Mikrobiologie, virologie |
| Klíčová slova | immobilization; DNase I; activity; stability; applications |
| Popis | The aim of work has been concentrated on degradation of high-weight and plasmid DNAs using immobilized DNase I. The activity of free and immobilized DNase I was estimated from UV spectrophotometric measurements and from agarose gel electrophoresis. The immobilized enzyme was full actived using Co2+ ions as an activator, a decrease of the activity was observed using other ions. Practical applications of DNase I immobilized on poly(HEMA-co-EDMA) microspheres in cleavage of plasmid DNAs are discussed. |
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