Informace o publikaci
SEM and AquaSEM studies of S. cerevisiae and C. moggii colonies
| Autoři | |
|---|---|
| Rok publikování | 2002 |
| Druh | Článek ve sborníku |
| Konference | Sborník XXXth Annual conference on yeasts |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Mikrobiologie, virologie |
| Klíčová slova | S. cerevisiae; C.moggii; yeast colonies; electron microscopy |
| Popis | Yeast colonies are organised multicellular structures arising from yeast cells growing on solid media only by means of cell division, thus resembling the formation of multicellular tissues of higher organisms. They exhibit organised morphologies, specific for particular yeast species. Regular structure of a colony should be achieved by interactions and signals between individual non-motile yeast cells, determining cell division polarity and their distribution during colony formation. With the aim to investigate the colony developmental processes, efficient and rapid methods of scanning electron microscopy of yeast colonies were established. These methods allowed us to monitor kinetics of a development of Saccharomyces cerevisiae and Candida mogii colonies, following two main morphological aspects: 1) morphology of the whole colony ('macroscopic morphology'), where 2 basic types of colonies (smooth and rough) were observed and 2) morphology of individual cells inside a colony ('microscopic morphology'). Cells in central and peripheral areas of colonies differed in their shape. In later phases, the oriented expansion of cells from peripheral parts of colonies to 'free space' was observed, indicating thus an effort of starving cells to growth towards areas with more nutrients. Formation of papillae growing up from the surface of 'older' smooth S.cerevisiae colonies might be another way of survival of cell subpopulations. The cells within colonies were connected by well-developed extracellular matrix. |
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