Informace o publikaci
A unique mechanism for protein processing and degradation in Arabidopsis thaliana
| Autoři | |
|---|---|
| Rok publikování | 2003 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Proceeding of the National Academy of Sciences of the USA |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://www.pnas.org/cgi/content/abstract/1230987100v1 |
| Obor | Genetika a molekulární biologie |
| Klíčová slova | vacuole; vacuolar processing enzyme; carboxypeptidase Y; invertase |
| Popis | Precursor protease vesicles are plant-specific compartments containing precursors of enzymes that are thought to participate in the degradation of cellular components in organs undergoing senescence. We report in vivo evidence that the precursor protease vesicle-localized vacuolar processing enzyme-{gamma} (VPE{gamma}) is critical for maturation of the plant vacuolar protease AtCPY. We also provide biochemical and functional evidence that VPE{gamma} is involved in degradation of the vacuolar invertase AtFruct4 in aging tissues. Moreover, a proteomics-based approach identified various proteins found in the vacuoles of aging vpe{gamma} mutants but not in WT plants, suggesting a unique role of VPE{gamma} in protein processing and degradation in Arabidopsis. |