Differential effects of c-Fos and v-Fos proteins on v-myb-transformed monoblasts

• Autoři: ZAHRADNÍČKOVÁ Eva; ŠEVČÍKOVÁ Sabina; SOUČEK Karel; ŠMARDA Jan
• Rok publikování: 2003
• Druh: Článek ve sborníku
• Konference: Memory in Living Systems
• Fakulta / Pracoviště MU: Fakulta informatiky
• Obor: Genetika a molekulární biologie
• Klíčová slova: Fos; Myb; proliferation; differentiation; apoptosis
• Popis: c-Fos and v-Fos proteins as well as v-Jun and c-Jun belong to the proteins forming the AP-1 transcription factor. The AP-1 participates in regulation of proliferation, differentiation and programmed cell death in multiple cell types. In this study we explored the effects of c-Fos and v-Fos proteins on the line of v-myb transformed chicken monoblasts BM2. Both c-Fos and v-Fos proteins were ectopically expressed in BM2 cells. The v-Fos protein induced growth arrest in G0G1-phase of the BM2 cell cycle similarly as c-Jun protein. Interestingly, c-Fos leaved the cell cycle unaffected and rather induced programmed cell death. This effect of c-Fos protein was markedly enhanced in cells cultivated under serum-free conditions. In contrast to Jun proteins, no differentiation promoting effect of c-Fos and v-Fos protein was detected in BM2 cells. These results suggest differential roles of individual components of the AP-1 transcription factor in regulation of differentiation and programmed cell.

Související projekty

• Úloha proteinů Fos a Jun v monocytické diferenciaci stimulované kyselinou retinovou
• Studium molekulárních mechanismů způsobujících supresi v-Myb s využitím přístupů proteomiky