Informace o publikaci
Determination of Midazolam from Perfuse Medium as a Probe Substrate of Cytochrome P450 3A1/2 Activity in Rats
| Autoři | |
|---|---|
| Rok publikování | 2004 |
| Druh | Článek ve sborníku |
| Konference | ACTA UNIVERISTATIS PALACKIANAE OLOMUCENSIS FACULTAS RERUM NATURALIUM |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Farmakologie a lékárnická chemie |
| Klíčová slova | Midazolam; CYP-450 |
| Popis | The aim of the project was to develop HPLC method for determination of midazolam and its three metabolites, 1- and 4-hydroxymidazolam and 1,4-dihydroxymidazolam from perfuse rat liver medium. Hydroxylation of midazolam is used as a valuable tool for measurement of metabolic activity of the 3A1/2 isoform of cytochrome P-450 in rats (CYP3A1/2)This method is simple, rapid, sensitive and applicable for modeling and description of possible pharmacological interactions on rat CYP3A1/2. |
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