Informace o publikaci

Evidence for the localization of the Arabidopsis cytokinin receptors AHK3 and AHK4 in the endoplasmic reticulum

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CAESAR Katharina THAMM Antje M. K. WITTHÖFT Janika ELGASS Kirstin HUPPENBERGER Peter GREFEN Christopher HORÁK Jakub HARTER Klaus

Rok publikování 2011
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of Experimental Botany
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://jxb.oxfordjournals.org/content/early/2011/08/12/jxb.err238.full.pdf+html
Doi http://dx.doi.org/10.1093/jxb/err238
Obor Genetika a molekulární biologie
Klíčová slova AHK3; cytokinin perception; endoplasmic reticulum; FIDSAM
Popis Cytokinins are hormones that are involved in various processes of plant growth and development. The model of cytokinin signalling starts with hormone perception through membrane-localized histidine kinase receptors. Although the biochemical properties and functions of these receptors have been extensively studied, there is no solid proof of their subcellular localization. Here, cell biological and biochemical evidence for the localization of functional fluorophor-tagged fusions of Arabidopsis histidine kinase 3 (AHK3) and 4 (AHK4), members of the cytokinin receptor family, in the endoplasmic reticulum (ER) is provided. Furthermore, membrane-bound AHK3 interacts with AHK4 in vivo. The ER localization and putative function of cytokinin receptors from the ER have major impacts on the concept of cytokinin perception and signalling, and hormonal cross-talk in plants.
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