LIQUID CHROMATOGRAPHY MASS SPECTROMETRY OF SELECTED BENZOPHENANTHRIDINE ALKALOIDS INCUBATED WITH RAT LIVER MICROSOMES
|Článek v odborném periodiku
|Časopis / Zdroj
|Fakulta / Pracoviště MU
|LC MS; QBA; benzophenanthridine; rat liver microsomes; metabolite
|Quaternary benzo[c]phenantridine alkaloids (QBAs) belong to a subgroup of isoquinoline alkaloids. They include major sanguinarine (SA) and chelerythrine (CHE) as well as minor QBAs chelirubine (CR), sanguilutine (SL), sanguirubine (SR), chelilutine (CL), and macarpine (MA). The effects of QBAs on biological systems have been studied extensively, especially for commercially available SA and CHE. Limited availability of the minor pentasubstituted QBAs and hexasubstituted MA reduces data concerning their biological effects. Although the QBA molecular structures are very similar, the mechanism of their action on the molecular level may be different. As pharmacological studies imply that even a small change in the structure may trigger a significant difference in pharmacological activity, it cannot be simply accepted that metabolic routes of all QBAs will be analogous. The metabolic fate of minor QBAs should be intensively studied in order to identify their metabolites, to reveal their toxicity and/or alternate biological effects in different pathways. An approach to reveal the metabolic fate of selected QBAs may employ an in vitro model of rat liver microsomes (RLMs) with resulted QBA metabolites to be analyzed by means of liquid chromatography (LC) coupled to hybrid quadrupole time-of-flight (Q-TOF) mass spectrometry (MS).