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Retinoid compounds associated with water blooms dominated by Microcystis species

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JAVŮREK Jakub SYCHROVÁ Eliška SMUTNÁ Marie BITTNER Michal KOHOUTEK Jiří ADAMOVSKÝ Ondřej NOVÁKOVÁ Kateřina SMETANOVÁ Soňa HILSCHEROVÁ Klára

Rok publikování 2015
Druh Článek v odborném periodiku
Časopis / Zdroj Harmful Algae
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www http://www.sciencedirect.com/science/article/pii/S1568988315000992
Doi http://dx.doi.org/10.1016/j.hal.2015.06.006
Obor Znečištění a kontrola vody
Klíčová slova Retinoic acid receptor; Cyanobacteria; Biomass extracts; All-trans-retinoic acid; 9-cis Retinoic acid; Retinoid-like activity
Popis Retinoic acids play a critical role in vital physiological processes and vertebrate development, their derivatives can be produced by some cyanobacterial species into surface waters. This study presents environmentally-relevant information on total retinoid-like activity of field cyanobacterial biomasses and surrounding waters. Intracellular and extracellular levels of total retinoid-like activity and retinoic acids have been investigated at a set of independent sites with the occurrence of water bloom species Microcystis aeruginosa. Twelve samples of biomass and surrounding water from seven localities affected by blooms were studied in comparison with samples from M. aeruginosa laboratory cultures. The method for biomass extraction was optimized and final extracts and samples of surrounding water concentrated by solid phase extraction were assessed using in vitro reporter gene bioassay and chemical analyses for all-trans-retinoic acid (ATRA), 9-cis retinoic acid (9-cis RA) and microcystins RR, LR and YR. Methanol was the most efficient solvent for the extraction of compounds with retinoid-like activity. An in vitro bioassay with the P19/A15 transgenic cell line revealed retinoid-like activity in all cyanobacterial biomasses in the range of 356-2838 ng of retinoid acid equivalents (REQ)/g dry mass (dm), while only three of surrounding water samples exhibited detectable retinoid-like activity, in the range of 12.8-28.7 ng REQ/L. Microcystins were detected in all samples, but they elicited no detectable retinoid-like activity up to 10 mg/L. Chemical analyses detected concentrations up to 340 ng/g dm of all-trans-retinoic acid (ATRA) and 84 ng/g dm 9-cis retinoic acid (9-cis RA) in bloom extracts, and up to 19 ng/L ATRA and 2.2 ng/L 9-cis RA in surrounding water. In most samples, ATRA and 9-cis RA contributed relatively little to the total REQs, which indicates the presence of significant amounts of other compounds with retinoic acid receptor-mediated modes of action.
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