Diplozoons (Monogenea): what is the molecular base of their interaction with the host?
|Fakulta / Pracoviště MU|
|Popis||The representatives from the class Monogenea are predominantly ectoparasites of fish. We adopted the experimental organisms - Eudiplozoon nipponicum (Diplozoidae, Heteronchoinea); a blood-feeding monogenean inhabiting gills of common carp (Cyprinus carpio). In contrast to the other groups of platyhelminths, the identity of genes, the level of their expression and spectrum of functional protein molecules produced by these parasites are very limited. The pyrosequencing methodology (454/Roche, MiSeq/HiSeq) enabled us to generate robust genomic/trancriptomic data and combined them with the results of comprehensive mass spectrometry analysis (Orbitrap-Elite MS System) of excretory-secretory products isolated from the adult worms. The pipelines of bioinformatic data processing were optimized and number of functional molecules was annotated. The reached platform currently represents significant source of information available for further experimental research; up to now at least six functional protein molecules (peptidases and their inhibitors) have been molecularly / biochemically / immunochemically characterized.|