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Rapid identification of staphylococci by Raman spectroscopy

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REBROŠOVÁ Katarína ŠILER M. SAMEK O. RŮŽIČKA Filip BERNATOVÁ S. HOLÁ Veronika JEŽEK J. ZEMÁNEK P. SOKOLOVÁ J. PETRÁŠ P.

Druh Článek v odborném periodiku
Časopis / Zdroj Scientific Reports
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1038/s41598-017-13940-w
Obor Mikrobiologie, virologie
Klíčová slova bacterium colony; human major clinical study; nonhuman ; Raman spectrometry; Staphylococcus
Popis Clinical treatment of the infections caused by various staphylococcal species differ depending on the actual cause of infection. Therefore, it is necessary to develop a fast and reliable method for identification of staphylococci. Raman spectroscopy is an optical method used in multiple scientific fields. Recent studies showed that the method has a potential for use in microbiological research, too. Our work here shows a possibility to identify staphylococci by Raman spectroscopy. We present a method that enables almost 100% successful identification of 16 of the clinically most important staphylococcal species directly from bacterial colonies grown on a Mueller-Hinton agar plate. We obtained characteristic Raman spectra of 277 staphylococcal strains belonging to 16 species from a 24-hour culture of each strain grown on the Mueller-Hinton agar plate using the Raman instrument. The results show that it is possible to distinguish among the tested species using Raman spectroscopy and therefore it has a great potential for use in routine clinical diagnostics.
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