Informace o publikaci
Effect of docosahexaenoic (DHA) and eicosapentaenoic acid (EPA) feeding on selected markers expression in rats
| Autoři | |
|---|---|
| Rok publikování | 2017 |
| Druh | Článek ve sborníku |
| Konference | MendelNet 2017 : Proceedings of 24th International PhD Students Conference |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | [online] |
| Obor | Chirurgie včetně transplantologie |
| Klíčová slova | rat; DHA; EPA; fatty acids; RT-PCR |
| Popis | The objective of the present study was to compare gene expression of selected markers depending on the type of diet with additional oil in feeding mixture. The experiment was analysed by the real-time polymerase chain reaction method (RT-PCR). As a housekeeping gene with stabile expression was selected beta-actin gene (Actb) and analysed markers were peroxisome proliferator- activated receptor gamma (PPARgamma), G-protein coupled receptor-sensor (GPR120), adiponectin receptor 1 (Adipor1) and adiponectin receptor 2 (Adipor2). Forty-eight adult male rats (Wistar Albino) were divided into four groups and were fed 10 weeks the diet containing specific oil for each group. Group R was fed by fish oil added to basic feeding mixture, group P was fed by palm oil added to basic mixture, group S was fed by safflower oil added to basic mixture and group D was fed by Schizochytrium microalga extract added to basic mixture. After 10 weeks of feeding, rats were sacrificed by isoflurane overdosing and liver samples were taken and expression of the liver genes coding four selected markers was measured. The main hypothesis of this project was if there were any differences in expression levels among groups depending on the type of diet. |