Informace o publikaci

Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells

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SOURALOVÁ Tereza HOLUBCOVÁ Zuzana KYJOVSKÁ Drahomíra HAMPL Aleš KOUTNÁ Irena

Rok publikování 2021
Druh Článek v odborném periodiku
Časopis / Zdroj Stem Cell Research
Citace
Doi http://dx.doi.org/10.1016/j.scr.2021.102574
Popis Human embryonic stem cells (hESCs) represent a virtually unlimited source of cells suitable for a variety of biomedical applications. However, a diminishing allogeneic background and undefined culture conditions are essential for developing robust and replicable protocols for differentiation experiments, disease modeling, and drug testing. Therefore, here we report the generation of the two sex-discordant sibling hESC lines, MUNIe008-A and MUNIe009-A, using the mechanical biopsy of vitrified-thawed embryos under xeno- and feeder-free conditions. The presented approach is applicable for deriving high-quality clinical-grade hESC lines for cell replacement therapies.
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