Colorectal Tumour Mucosa Microbiome Is Enriched in Oral Pathogens and Deﬁnes Three Subtypes That Correlate with Markers of Tumour Progression
|Článek v odborném periodiku
|Časopis / Zdroj
|Fakulta / Pracoviště MU
|colorectal cancer; 16S rRNA gene; tumour microbiome; microbial subtypes
|Dysbiosis of the gut microbiome may contribute to the heterogeneity of colorectal cancer from phenotypic, prognostic and response to treatment perspectives. We analysed CRC microbiome by 16S rRNA gene sequencing of paired tumour mucosa, adjacent visually normal mucosa and stool swabs of 178 patients with stage 0–IV CRC. We observed that tumour mucosa is dominated by pathogenic bacteria of oral origin and proposed a CRC tumour microbiome subtyping system. The subtypes and tumour mucosa genera were associated with prognostic clinical covariates (tumour grade, localisation, TNM, BRAF mutation and MSI). In contrast, changes in the stool microbiome were associated with lymph node involvement and the presence of synchronous metastases. We discovered new associations between microorganisms and CRC and clinical parameters. Our study represents a step forward in understanding the role of the microbiome and its interactions with factors involved in tumour progression, and it opens novel avenues for exploring new treatments and biomarkers.