Informace o publikaci

Detection and measurement of fungal burden in a guinea pig model of invasive pulmonary aspergillosis by novel quantitative nested real-time PCR compared with galactomannan and (1,3)-beta-D-glucan detection.

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LENGEROVÁ Martina KOCMANOVÁ Iva RÁČIL Zdeněk HRNČÍŘOVÁ Kristýna POSPÍŠILOVÁ Šárka MAYER Jiří NAJVAR Laura K WIEDERHOLD Nathan P KIRKPATRICK William R PATTERSON Thomas F

Druh Článek v odborném periodiku
Časopis / Zdroj Journal of Clinical Microbiology
Fakulta / Pracoviště MU

Lékařská fakulta Přírodovědecká fakulta Středoevropský technologický institut

Citace
WWW http://www.ncbi.nlm.nih.gov/pubmed/22189110
Doi http://dx.doi.org/10.1128/JCM.05356-11
Obor Onkologie a hematologie
Klíčová slova Invasive pulmonary aspergillosis; real-time PCR; galactomannan; glucan
Popis We developed and assessed the diagnostic value of a novel quantitative nested real-time (QNRT) PCR assay targeting the internal transcribed spacer region of ribosomal DNA (rDNA) in a guinea pig model of invasive pulmonary aspergillosis. Groups of 5 immunosuppressed animals that were infected using an aerosol chamber with Aspergillus fumigatus conidia were humanely terminated 1 h postinoculation and at days 3,5,7,and 11 postchallenge, and lung tissue, bronchoalveolar lavage (BAL) fluid, whole blood, and serum samples were collected. The QNRT PCR results obtained with the serum and BAL fluid were compared to those achieved with galactomannan and (1,3)-beta-D-glucan assays. High fungal burden levels were detected by QNRT PCR in both lung tissue and BAL fluid in all infected animals at each time point, and the sensitivity of each assay in BAL fluid was 100% by day 3 and remained so through the remainder of the study. The sensitivity of detection of fungi in whole blood and serum samples was significantly lower, and some samples remained negative by all three assays despite the advanced stage of the infection. From these data, we can conclude that this novel QNRT PCR method was highly sensitive for the detection of A. fumigatus from different types of samples in this model. In addition, BAL fluid samples appeared to be the most suitable for the early diagnosis of invasive pulmonary aspergillosis. When testing serum, the use of a combination of available assays may increase the possibility of early detection of this opportunistic mycosis.
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