Informace o publikaci
Immobilization of Haloalkane Dehalogenase LinB from Sphingobium japonicum UT26 for Biotechnological Applications
| Autoři | |
|---|---|
| Rok publikování | 2013 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Journal of Biocatalysis & Biotransformation |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Biochemie |
| Klíčová slova | Haloalkane Dehalogenase |
| Popis | Haloalkane dehalogenases are enzymes capable of converting a broad range of aliphatic halogenated compounds to corresponding alcohols. These dehalogenase-based biotransformations are attractive for various biological processes, e.g. biocatalysis, bioremediation and detoxification, which often require protein immobilization. Different immobilization techniques, including (i) cross-linking using glutaraldehyde, dextran polyaldehyde, disuccinimidyl suberate and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, (ii) formation of cross-linked enzyme aggregates, and (iii) entrapment methods using organically modified sol-gel ORMOCER and polyvinyl alcohol particles, were systematically investigated for immobilization of selected haloalkane dehalogenase LinB from Sphingobium japonicum UT26. |
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