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A new approach of antibodies labeling (NPs) by immunoassay with LA-ICP-MS detection

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TVRDOŇOVÁ Michaela ASCHER Lena JAKUBOWSKI Norbert VLČNOVSKÁ Marcela VACULOVIČOVÁ Markéta KANICKÝ Viktor VACULOVIČ Tomáš

Rok publikování 2018
Druh Konferenční abstrakty
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Popis Laser ablation with inductively coupled plasma is still more used in life science as biology and biomedicine and the utilization of metals and proteins determination simultaneously is also growing up. 1,2 We have developed a new strategy of antibody labeling (it can specific binds to proteins) by Au nanoparticles which are composed of thousands of atoms and thus increase the sensitivity enormously and decrease the limit of detection in comparison to lanthanoids labeling. The ability of successfully labelled antibodies bounded to antigen (protein) was proved by dot blot on the membrane and the Au was imaged by LA-ICP-MS. One of the many possible applications is binding of labelled antibodies to the tumour tissues containing metallothionein (known as a transporter of Cu, Zn and heavy metals) and image their distribution in different tumour stage.
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