Novel buffer screen for biomolecular characterization
|Fakulta / Pracoviště MU
|The protein characteristics are heavily influenced by the solution nature, however, the determination of a suitable buffer environment for a protein of interest is not an easy task. The requirements of advanced techniques, the demands on the biological material, and the researcher’s time needed for buffer optimization, as well as personal inflexibility, lead frequently to the use of sub-optimal buffers. Here, we demonstrate the design of a 48-condition buffer screen that can be used to determine an appropriate environment for downstream studies.