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Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry

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TVRDOŇOVÁ Michaela VLČNOVSKÁ Marcela VANICKOVA Lucie KANICKÝ Viktor ADAM Vojtěch ASCHER Lena JAKUBOWSKI Norbert VACULOVIČOVÁ Markéta VACULOVIČ Tomáš

Rok publikování 2019
Druh Článek v odborném periodiku
Časopis / Zdroj Analytical and Bioanalytical chemistry
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www http://dx.doi.org/10.1007/s00216-018-1300-7
Doi http://dx.doi.org/10.1007/s00216-018-1300-7
Klíčová slova Dot blot; Antibody labelling; Gold nanoparticles; LA-ICP-MS; Immunochemistry
Popis In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.
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